ENGITEIN

Custom Protein Production

High Yield AI Structure Prediction Protein Design

We produce high-yield recombinant proteins using a wide range of E. coli expression systems and optimized culture conditions.

From gene synthesis to protein purification, our end-to-end workflow delivers high-purity proteins suitable for research reagents and diagnostic applications at a cost-effective price.

Expression Hosts BL21(DE3), Rosetta(DE3)

Vectors & Tags License-free pET series / His, GST, MBP, SUMO

Target Purity 90–95% or higher (SDS-PAGE)

Production Scale From lab scale (1–100 mg) to pilot scale (gram level)

Quality Control SDS-PAGE, Bradford Assay, UV Quantification, Optional

Western Blot

Lead Time 2–3 weeks after sequence confirmation

(excluding gene synthesis)

Inclusion Body Refolding

Insoluble Refolding Disulfide Bonding

For proteins expressed as insoluble inclusion bodies, ENGITEIN applies its proprietary refolding technology to recover soluble, biologically active proteins.

Our process restores native protein structure and bioactivity, providing reliable solutions for proteins that are difficult to obtain using conventional methods.

Target Protein Insoluble Inclusion Bodies

Refolding Method Engitein's Special Method

Final Product Soluble, Biologically Active Protein

Buffer Options PBS, Tris-HCl, or Customer-Specified Buffer

Additional Analysis SEC-HPLC (Aggregation Analysis), Activity Assay

Lead Time 4–8 weeks (including process optimization)